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KMID : 0363420120260010001
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Journal of Korean Oriental Pediatrics
2012 Volume.26 No. 1 p.1 ~ p.15
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Suppressive Effect of CheongGiGeoYangTang on Allergic Inflammation of RBL-2H3 Mast Cells and OVA/alum-sensitized Mice
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Lee Hye-Lim
Han Jae-Kyung
Kim Yun-Hee
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Abstract
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CheongGiGeoYangTang has been used for anti-allergenic purpose. However there was no experimental study about its effect. Therefore, this study was designed to investigate the anti-allergenic effect of CheongGiGeoYang.
Methods: Modifiability of RBL-2H3 mast cells’ IL-4, IL-13 was analyzed by qRT-PCR and ELISA. Also, the suppressive effect of GATA-1, GATA-2, NF-AT1, NF-AT2, AP-1 and NF- ĸβ p65 transcription factors was observed by western blotting. OVA-specific IgE, IL-4 and IL-13 production in ovalbumin allergy model was examined as well.
Results: It was showed that the RBL-2H3 mast cells treated with CheongGiGeoYang extract(CGGYT) was significantly suppressed mRNA expression, production of IL-4 and IL-13, and prominently inhibited the expression of transcription factors including GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos and NF- ĸβ p65 but not c-Jun. The administration of CGGYT was suppressed the amount of OVA-specific IgE, IL-4 and IL-13 in OVA/alum-sensitized mice.
Conclusions: We considered CGGYT would regulate the allergic inflammation as inhibition of IL-4 and IL-13 production in activated mast cells and Th2 cells.
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KEYWORD
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CheongGiGeoYangTang(淸肌祛痒湯), RBL-2H3 mast cell, OVA/alum-sensitized mice, IL-4, IL-13, Anti-allergic
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